Metabolomic characterization of strawberry cultivars during postharvest

The cultivated strawberry (Fragaria x ananassa) is the berry most consumed worldwide and is well-known for its delicate flavour and nutritional characteristics. However, strawberries possess a very short postharvest shelf-life due to their high respiration rate and their susceptibility to water loss, mechanical damage and fungi deterioration. Extension of fruit shelf-life is a major economic goal, and measures are commercially taken to delay senescence. These procedures include low temperature, controlled atmosphere and/or chemical treatments, being the first one the most commonly applied. To improve our understanding of the molecular and biochemical mechanisms underlying the deterioration of fruit quality attributes during senescence, we monitored the metabolomic profiles of five commercial strawberry cultivars under different postharvest treatments. Ripe fruits of F x ananassa cv. ‘Amiga’, ‘Camarosa’, ‘Candonga’, ‘Fortuna’ and ‘Santa Clara’ were harvested and kept at 4ºC during three, six and ten days in normal, CO2-enriched and O3-enriched atmospheres. We used a combination of GC-TOF-MS, LC-MS and GC-SPME-MS to identify and semi-quantify 49 primary metabolites (sugars, amino and organic acids), 132 polar secondary metabolites and 70 volatile compounds in all different treatments along postharvest stages. Multivariate statistical approaches, including hierarchical cluster analysis, partial least squares discriminant analyses and k-means clustering, were used to characterize the variation in metabolite content during the strawberry fruit postharvest life and to identify the biochemical pathways which are most affected in the senescence processes. Here, we present the main changes in volatile compounds, primary and secondary metabolites as a consequence of postharvest storage, highlighting the differences between cultivars and treatments. Network-based methods will allow us to point out the regulatory factors and molecular mechanisms underlying fruit senescence.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Analysis of magnetic and structural properties in La0.6Sr 0.4MnO3 ferromagnetic particles under the influence of mechanical ball milling effect

We have investigated the magnetic, structural and morphological properties of La0.6Sr0.4MnO3 (LSMO-40) manganite particles, synthesized by solid state reaction method. The resulting LSMO-40 powders were milled in air atmosphere during 3, 6 and 12 hours, by using a planetary ball milling. Samples obtained were characterized by X-ray diffraction, scanning electron microscopy – SEM and magnetization measurements as a function of temperature and magnetic field. A Rietveld analysis was carried on each XRD pattern, and was observed a reduction in crystallite average size (Dv) with increased ball milling time, tM. This is associated with a decrease in particle size. A characteristic rhombohedral crystal structure for the LSMO-40 phase was identified (space group R3C), independent of the milling time of the powders. However, from SEM microstructure was observed more homogeneity in the grain distribution by milling process. The results of magnetic characterization, showed that samples with higher tM (smaller grain size), presented the lowest value of the saturation magnetization, which is attributed to surface effects that induce magnetically disordered states with decreasing particle sizes. This magnetic anisotropy surface is evidenced also on the changes of coercive fields, HC, measured at low temperatures, which increased with increasing tM

A New Pleiades Member at the Lithium Substellar Boundary

We present the discovery of an object in the Pleiades open cluster, named Teide 2, with optical and infrared photometry which place it on the cluster sequence slightly below the expected substellar mass limit. We have obtained low- and high-resolution spectra that allow us to determine its spectral type (M6), radial velocity and rotational broadening; and to detect H$_\alpha$ in emission and Li I 670.8 nm in absorption. All the observed properties strongly support the membership of Teide 2 into the Pleiades. This object has an important role in defining the reappearance of lithium below the substellar limit in the Pleiades. The age of the Pleiades very low-mass members based on their luminosities and absence or presence of lithium is constrained to be in the range 100--120 Myr.Comment: 17 pages, 3 figure

Search and characterization of T-type planetary mass candidates in the sigma Orionis cluster

(Abridged) We aim to: i) confirm the presence of methane absorption in S Ori 73 (a T-type member candidate of the sig Orionis cluster, 3 Myr, 352 pc) through methane imaging; ii) study S Ori 70 and 73 cluster membership via photometric colors and accurate proper motion analysis; iii) perform a new search to identify additional T-type sig Orionis member candidates with likely masses below 7 Mjup. We obtained HAWK-I (VLT) J, H, and CH4off photometry of an area of 119.15 sq. arcmin in sig Orionis down to Jcomp = 21.7 and Hcomp = 21 mag. Near-infrared data were complemented with optical photometry using images acquired with OSIRIS (GTC) and VISTA as part of the VISTA Orion survey. We derived proper motions by comparison of the new HAWK-I and VISTA images with published near-infrared data taken 3.4 - 7.9 yr ago. S Ori 73 has a red H-CH4off color indicating methane absorption in the H-band and a spectral type of T4 +/- 1. S Ori 70 displays a redder methane color than S Ori 73 in agreement with its latter spectral classification. Our proper motion measurements are larger than the motion of sig Orionis, rendering S Ori 70 and 73 cluster membership uncertain. We identified one new photometric candidate with J = 21.69 +/- 0.12 mag and methane color consistent with spectral type greater than T8. S Ori 73 has colors similar to those of T3-T5 field dwarfs, which in addition to its high proper motion suggests that it is probably a field dwarf located at 170-200 pc. The origin of S Ori 70 remains unclear: it can be a field, foreground mid- to late-T free-floating dwarf with peculiar colors, or an orphan planet ejected through strong dynamical interactions from sig Orionis or from a nearby star-forming region in Orion.Comment: Accepted for publication in A&

The balance between fumarate and malate plays an important role in plant development and postharvest quality in tomato fruit

Organic acids, produced as intermediates of the tricarboxylic cycle, play a crucial role in the plant primary metabolism and are considered as being ones of the most important quality traits in edible fruits. Even if they are key metabolites in a multitude of cellular functions, little is known about their physiological relevance and regulation. Transgenic tomato (Solanum lycopersicum) plants expressing constitutively a bacterial maleate isomerase, which converts reversibly maleate to fumarate, were generated in order to improve our knowledge about the role of organic acids in the crop and fruit metabolism. Growth and reproduction were affected by the unbalance of tricarboxylic cycle intermediates, as a dwarf phenotype and a flowering delay were observed in the transgenic plants. In addition, a delay in chlorophyll synthesis, a decrease in the numbers of stomata and significant changes in some photosynthetic parameters indicated alterations in central primary metabolism. Postharvest was also impaired, as transgenic fruits showed increased water lost and deterioration, indicating a possible role of the organic acids in cell wall metabolism. Finally, preliminary metabolomics analysis pointed out important changes during fruit ripening in flavor-related metabolites, such as acids and sugars, revealing the importance of organic acids in fruit metabolism. Taken together, these data indicate a pivotal role of tricarboxylic cycle intermediates, such as malate or fumarate, as regulatory metabolites. Besides their role in quality fruit characteristics, they are involved in a multitude of functions including growth and photosynthesis.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

A note on Influence diagnostics in nonlinear mixed-effects elliptical models

This paper provides general matrix formulas for computing the score function, the (expected and observed) Fisher information and the $\Delta$ matrices (required for the assessment of local influence) for a quite general model which includes the one proposed by Russo et al. (2009). Additionally, we also present an expression for the generalized leverage. The matrix formulation has a considerable advantage, since although the complexity of the postulated model, all general formulas are compact, clear and have nice forms.Comment: Paper submitted for possible publication, 6 page